Research Scientist – Protein engineering

Kyiv | 03 февраля 2023
Тип занятости: полная занятость

Yuria-Pharm Corporation is an international pharmaceutical corporation, one of the most dynamic and fast-growing pharmaceutical manufacturers of Ukraine. The Corporation's strategic priority is the implementation of advanced technologies for the development and production of pharmaceutical and biopharmaceutical APIs and drugs based on them. We strive to provide patients with access to the highest quality infusion therapy, highly effective targeted therapy, vaccines and therapeutic solutions for combating orphan diseases.

The R&D center of Yuria-Pharm has world-class R&D infrastructure, which allows to carry out a full range of research, development and pilot production of medicinal products based on small molecules, recombinant proteins and mRNA. We have created a unique environment where scientists are able to work on the development of solutions that change lives of millions of patients worldwide.

Our corporate R&D center in Kyiv, Ukraine invites you to join our team as a Research Scientist working on the development of novel vector systems and technologies for eukaryotic recombinant protein engineering and expression.

Responsibilities:

  • Development and optimization of technologies for the generation of eukaryotic recombinant protein-producing cell lines (CHO-based), intended for cGMP manufacturing of recombinant protein therapeutics
  • Design, assembly and preparation of vector constructions/vector libraries for recombinant proteins expression using bacterial/eukaryotic expression systems
  • Lab-scale synthesis and purification of recombinant proteins, intended for research use.
  • Design, assembly and characterization of native, immune and synthetic high-diversity scFv- and FAB-libraries using filamentous phage display platform
  • Sequence engineering/optimization of recombinant proteins
  • Development, qualification and validation of analytical procedures, based on PCR/qPCR, sequencing (Sanger/NGS), slab and capillary electrophoresis, nucleic acid/protein blotting etc. Analytical support of research and development activities utilizing the developed methods.
  • Documentation of experiments results using ELN, review and validation of team experiment records, preparation of presentation materials/presentation of results to the stakeholders
  • Preparation of documentation for regulatory submission as a part of the M3 CTD dossier for a drug product
  • Support of the research group maintenance activities (materials and equipment management, new employees training, preparation and submission of SOPs etc.).

Basic qualification:

  • BS degree in biology, biotechnology
  • 2+ year of experience including the following:
  • - academic research involving hands-on experience with design, assembly and QC of plasmid-based expression vectors/vector libraries for recombinant protein expression in bacterial/eukaryotic expression systems (HEK293, CHO, Sp2/0 preferable)
- hands-on experience with application and optimization of the basic molecular biology methods and technics including agarose slab gel electrophoresis, PCR/qPCR, plasmid/genomic DNA/RNA isolation, transformation/transfection)
  • Basic scripting/programming skills (Python/BASH is required)
  • Language knowledge requirements: English (upper-intermediate).

Preferred skills and experience:

  1. PhD in biochemistry, biophysics, molecular biology, immunology
  2. Practical experience with the following analytical technics: Agarose/PAGE slab electrophoresis, CE/UV, CE/LIF, Blotting technics (Northern Blot, Western Blot), PCR/qPCR/dPCR, NGS DNA sequencing (OxNano/Illumina), Spectrometry/Fluorimetry
  3. Experience in planning and performing complex genetic assemblies using “scar-free” cloning technics (Gibson cloning, “Golden Gate” cloning, LIC etc.)
  4. Experience with tools, that enable design/filtration/preparation for submission of synthetic nucleotide libraries
  5. Practical experience with troubleshooting/optimization of bacterial expression for recombinant proteins (multi-subunit/toxic proteins expression)
  6. Protein purification experiences using His-tag/StrepTag II affinity tagging
  7. Experiment design and data processing for the HT experimental data (RNAseq, ATAC-seq)
  8. Knowledge of API for the key bioinformatics databases interfacing (GenBank, ENCODE, GO, OMIM etc).
  9. Firm understanding and experience in application of enzyme kinetics theory for characterization and engineering of enzymes
  10. Practical experience in programming/operation/maintenance of liquid handing robotics systems (Opentrons/Tecan preferable)
  11. Practical understanding of DoE methodology, experience with the data processing tools, suitable for multivariate data analysis (ex Statfoft Statistica, SPSS)
  12. Solid background in statistical concepts, applicable to life science research
  13. Practical experience with analytical method validation according to ICHQ2A guidelines, documentation of validation process in regulated environments (ISO 17025-certified facilities)
  14. Demonstrated ability to drive complex projects to completion.

Special requirements:

  • Self-motivation with strong skills for provisional and operational planning, multitasking
  • The ability to adapt to work in the dynamic development environment
  • Ability to work overtime to meet required deadlines
  • The ability to work in a team and organize the work of teams
  • Accurate record keeping and attention to details in records maintenance.

We offer:

  • A unique world-class integrated R&D platform, enabled with state-of-the-art infrastructure, that provides a full range of opportunities for the implementation of research programs of any complexity
  • The opportunity to work on the most advanced bioprocess platform in Ukraine
  • The possibility of participation in internships and professional development programs, publication of scientific works, patents.
  • A comfortable corporate campus in Kyiv
  • Lunches provided for employees of the laboratory complex
  • Flexible work schedule with the possibility of shifting working hours.
  • 30 days of paid vacation annually
  • Employee special discounts program for the medicines and products of internal manufacturing.
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